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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Plant Growth Regulators and Explant Types on Callus Formation in African Yam Bean (Sphenostylis stenocarpa (Hochst. Ex A. Rich) Harms)) ผู้เขียน: Sikirat Remi Akande, Morufat Oloruntoyin Balogun, Benjamin Adefemi Ogunbodede สื่อสิ่งพิมพ์:pdf AbstractThis study was conducted to investigate the effect of explant types and plant growth regulator regimes on callus induction in African yam bean, with a view to developing protocols for regeneration. Leaf, stem and root explants of African yam bean were cultured in media containing different types and concentrations of plant growth regulators (PGR). The explants were observed for callus formation after three months. The main effects of variety, explant and plant growth regulator regimes and their interactions were highly significant. Highest callus formation was recorded in stem explant while the root explant had the lowest. The accession, SSSWN56 (brown seed) had higher percentage callus formation than SSSWN75 (grey seed). No callus was formed in hormone-free medium and the media containing indole acetic acid (IAA). One- hundred-percent callus formation was recorded for both accessions in the medium containing 1.5 mg/l each of kinetin and Naphthalene acetic acid (NAA). This medium is recommended for callus induction using stem explant and it will be useful in perfecting regeneration protocols for African yam bean. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Regeneration of African Yam Bean (Sphenostylis stenocarpa (Hochst ex. A. Rich.) Harms by Direct Organogenesis) ผู้เขียน:Adesoye A.I., Emese A., Olayode O.M. สื่อสิ่งพิมพ์:pdf AbstractStudies on in vitro organogenesis of African yam bean (Sphenostylis stenocarpa) were carried out with the aim of developing a rapid regeneration system for this crop. Embryo and leaf explants were cultured on Murashige and Skoog (MS) containing varying concentrations and combinations of 6-benzyl aminopurine (BAP), kinetin and ?-naphthalene acetic acid (NAA). The maximum number of shoots per explant (4.5) and percentage multiple shoot induction (100%) were obtained in MS supplemented with 0.05 mg.L-1 NAA and 0.5 mg.L-1 BAP. The maximum shoot length (135 mm) was obtained on a medium with 1.0 mg.L-1 kinetin and 0.1 mg.L-1 NAA. When cotyledonary node explants and shoot tip explants were cultured on media with BAP and kinetin singly, each at 1.0, 1.5 and 2.0 mg.L-1, both explants produced the maximum number of shoots (4.75) and shoot length (25 mm) on 2.0 mg.L-1 BAP while the least responses were obtained on 1.0 mg.L-1 kinetin. There was no organ formation from leaves as they all produced calli. Multiple shoots from the embryo produced roots directly on shoot induction medium while shoot tip-derived multiple shoots rooted when tested on both 0.25 and 0.5 mg.L-1 NAA. Shoots from cotyledonary nodes did not produce roots. Successfully rooted plantlets obtained from this study is the fi rst report of in vitro plant regeneration in African yam bean. This procedure for direct organ differentiation would facilitate micropropagation and improvement of this species through genetic transformation. |
 ที่มา:The first Mae Fah Luang University International Conference 2012 on ASEAN Integrationหัวเรื่อง:การผลิตเอธานอลจากมันแกวโดยใช้เชื้อยีสต์ Saccharomyces cerevisiae TISTR 5339 |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Performance, Hematology and Serum Biochemistry of West African Dwarf Goats Fed Ensiled Mixtures of Elephant Grass (Pennisetum purpureum) with Lima Bean, African Yam Bean and ผู้เขียน:Ajayi Festus Tope, Ogunleke Funmi, Adesina Adewumi, Durotoye Emmanuel Sunday สื่อสิ่งพิมพ์:pdf AbstractThe performance, hematological and biochemical parameters of 16 West African dwarf goats fed ensiled elephant grass (Pennisetum purpureum) with pineapple pulp (Diet 1), pigeon pea plant (Diet 2), lima bean plant (Diet 3) and African yam bean plant (Diet 4) were investigated in a completely randomized design. Four goats were allotted to each diet in a 91-day feeding trial; each goat was fed at 5% of its body weight. The results showed that the crude protein, ether extract and ash contents of Diets 2–4 were similar (P > 0.05). The dry matter intake, crude protein intake and neutral detergent fi ber intake of goats fed Diet 3 differed signifi cantly (P < 0.05) from that of goats fed Diets 1, 2 and 4 which were 71.2, 28.8 and 52.7 g per kilogram W0.75 per day (metabolic weight per day), respectively. The highest packed cell volume was observed in goats on Diet 2 (29.3%) and differed signifi cantly (P < 0.05) from that of goats fed Diets 1, 3 and 4. Goats fed Diet 3 had the highest hemoglobin concentration, lymphocyte, monocytes and eosinophils. Goats fed Diet 4 had the highest total protein (70.2 g.L-1) and cholesterol (42.0 mmol.L-1).The highest urea nitrogen (3.8 mmol.L-1), creatinine 97.0 ?mol.L-1), AST (51.9 IU.L-1), ALT (6.7 IU.L-1), glucose (3.1 mmol.L-1) and lowest cholesterol (31.2 mmol.L-1) were observed in goats fed with Diet 3. These results indicated that ensiling elephant grass with any of these legumes known to contain high concentrations of anti-nutrients was not detrimental to the health of the goats. Diet 3, comprising ensiled elephant grass with lima bean and pineapple pulp, produced the optimal growth rate and weight gain in the goats. |
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 Researcherดร. รัชพล พะวงศ์รัตน์, ผู้ช่วยศาสตราจารย์ที่ทำงาน:ภาควิชาวิทยาศาสตร์และนวัตกรรมชีวภาพ คณะศิลปศาสตร์และวิทยาศาสตร์ สาขาที่สนใจ:Enzyme Technology, Bioremediation Technology , Biotechnology, Lipids Technology, Application of Enzyme in Lipid Modification, Bioremediation Technology, Biodiesel Resume |
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